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| Year | Event |
| 1668 | Francesco Redi attacks spontaneous generation and disproves it for large organisms |
| 1745 | John Needham adds chick broth to a flask and boils it, lets it cool and waits. Microbes grow and he proposes it as an example of spontaneous generation. |
| 1768 | Lazzaro Spallanzani repeats Needham's experiment, but removes all the air from the flask. No growth occurs. |
| 1859 | Louis Pasteur's swan-neck flasks show that spontaneous generation does not occur. |
| 1870 |
Thomas H. Huxley gives his "Biogenesis and Abiogenesis" lecture. The speech offered powerful support for Pasteur's claim to have experimentally disproved spontaneous generation. |
| 1877 | John Tyndall publishes his method for fractional sterilization, showing the existence of heat-resistant bacterial spores. |