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[Prev] | [Next]For years the existence of microorganisms was suspected, but could not be proven, since bacteria were too small to be seen with the naked eye. It took the microscope to expose their tiny world and that instrument has been linked to microbiology ever since. In 1664, Robert Hooke devised a compound microscope and used it to observe fleas, sponges, bird feathers, plants and molds, among other items. His work was published in Micrographia and became a popular and widely read book at the time.
Several years later Anton van Leeuwenhoek, a fabric merchant and amateur scientist (or "natural philosopher" as such people called themselves), became very adept at grinding glass lenses to make telescopes and microscopes. While crude by modern standards, his were a technical marvel for the time, able to magnify samples greater than 200-fold - a reproduction of a Leeuwenhoek microscope is shown in Figure 1-3. They also produced clearer images than the compound microscopes of the time. By peering through his microscope, Leeuwenhoek observed tiny organisms or "wee animacules" as he called them. He spent months looking at every kind of sample he could find and eventually submitted his observations in a letter to the Royal Society of London, causing a sensation. Hooke was asked to confirm the findings of Leeuwenhoek and his affirmative assessment garnered them wide acceptance. Surprisingly the work of these two scientists was not followed up for almost 200 years. Human societies had neither the technical prowess nor the inclination to develop the science of microorganisms. It was not until the rise of the industrial revolution that governments and people dedicated the financial and physical resources to understand these small inhabitants of our world.

The left panel shows a replica of a Leeuwenhoek microscope . The photomicrographs in the center and right were taken in the early 20th century through one of Leeuwenhoek's microscope. (Source: The Leeuwenhoek Letter. Society of American Bacteriologists. Baltimore. 1937.)
With the development of better microscopes in the 19th century, scientists returned to an examination of microorganisms. After finishing his education, Ferdinand Julius Cohn was able to convince his father to lay down the large sum necessary to purchase a microscope for him, one better than that available at the University in Breslau, then part of Germany. He used it to carefully examine the world of the microbe and made many observations of eukaryotic microorganisms and bacteria. His landmark papers on the cycling of elements in nature was published in Ueber Bakterien in 1872 and a microbial classification scheme including descriptions of Bacillus were published in the first volume of a journal he founded, Beitraege zur Biologie der Planzen. Cohn's work with microscopes popularized their use in microbiology. This and his other work inspired many other scientists to examine microbes. Cohn's encouragement of Robert Koch, a German physician by training, began the field of medical microbiology.
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